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KMID : 0380020150300040191
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 4 p.191 ~ p.196
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Anthocyanin Analysis of Pressure-extracted Korean Blueberry Juice and in vitro Anti-inflammatory in RAW267.4 Cell line
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Choi Moon-Hee
Jeon Young-Jin
Shin Hyun-Jae
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Abstract
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Blueberry juice possesses rich-procyanidins and - anthocyanidin, comprised a group of with numerous health benefits such as protection against coronary heart disease, detoxification, and obesity. Blueberry (Vaccinium virgatum) juice extracts were analyzed and separated by an HPLC method for the purpose of the separation and quantification in polyphenolic groups. In specific HPLC conditions, a binary mobile phase consisting of formic acid: water (10:90, v/v, solvent A) and formic acid: water: acetonitrile (10:60:30, v/v/v, solvent B) was utilized and it is detected at 546 nm wavelength. The phenolic contents of the extracts are determined using Folin-Ciocalteu phenol reagent. In order to test anti-inflammation activity assay, after producing nitric oxide (NO) in lipopolysaccharide activated RAW 264.7 cells, at concentration of 20-500 μg/mL it reduced to NO production at a dose-dependent manner. Importantly, cytotoxicity assay with up to 500 μg/mL of the extract from blueberry juice showed ~100% cell viability for RAW264.7 cell line. Therefore, Korean blueberry juice might have potential as anti-oxidant and antiinflammation agents.
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KEYWORD
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Blueberry, Anthocyanin, Polyphenol, Nitric oxide, Cytotoxicity, Antioxidant activity
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